This publication is a microbiology laboratory manual designed for a one-semester, college undergraduate education. The manual is designed to be self-guided so that the students can learn and discover on their own time. All college majors requiring a microbiology laboratory course will benefit from this publication. This manual contains a series of experiments designed to build a student's knowledge and mastery of microbiological laboratory techniques. It begins with basics of microscopy, aseptic technique, and culture handling, and then advances into diagnostic techniques applicable to many fields in microbiology. In addition, the manual has an unknown exercise that follows the student through the entire semester allowing for them to use various techniques and a dichotomous key to determine the identity of microorganisms.
- Lab 1. Introduction To Microscopy
- Lab 2. Handwashing
- Lab 3. Aseptic Technique And Pure Culture Transfers
- Lab 4. Simple Stain
- Lab 5. Fungi
- Lab 6. The Gram Stain
- Lab 7. Serial Dilutions (Viable Plate Counts)
- Lab 8. Antimicrobial Susceptibility
- Lab 9. Metabolism
- Lab 10. Metabolism 2 And Motility
- Lab 11. Selective And Differential Media
- Lab 12. Food Microbiology
- Lab 13. Water Microbiology
- Lab 14. Identification Of An Unknown Bacterial Species
- Appendices
